Nanocarried antioxidants in freezing extenders for boar spermatozoa

Post-thawing cryoinjuries in boar spermatozoa due to oxidative stress may be reduced by adding nanoencapsulated antioxidants freezing extenders. This study evaluated post-thawing kinetics, structural and biochemical functions of frozen with extenders including resveratrol vitamin E loaded into polymeric nanocapsules. Resveratrol was added at 0 (control), 5, 10, 20, 40 80 µg/ml, whereas Vitamin 50, 100, 200 400 µg/ml. Both were tested free presentations. In contact empty nanocapsules, some sperm kinetics parameters impaired compared the control (p < .05), lipoperoxidation declined .05). With inclusion µg/ml resveratrol, improved .01), but motility, did not differ from > No improvement quality occurred E, although .01). Inclusion benefitted after thawing, no resulted E.